Part:BBa_K4653103:Design

3xFlg22

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 97
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 175
  
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

We repeated the sequence of Flg22 three times to increase the probability of our PAMP reaching the plant pattern recognition receptor to enhance the plant immune response.

Source

Flg22 a 22-amino acid sequence from the N-terminal of flagellin from P. aeruginosa，We repeated the sequence of Flg22 three times to increase the probability of our PAMP reaching the plant pattern recognition receptor to enhance the plant immune response.